Freezing Cells: Cell Culture Basics

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L929?

How do you determine the appropriate volume of freezing media?

is this necessary to filter the freezing medium?

Addition of cryoprotectant in freezing cell (like DMSO) is missing.

Can we preserve cells in -70 C for long term storage?

What is the freezing media?

i freezing cells in media of (10 %DMSO + 10%FBS + my media ( in case of 293hACE2 for exa. 100µ DMSO ; 100µ FBS + 800 DMEM media) ..

How do you know when your cells are ready to be frozen? Is it at a specific passage number?

Did you use PBS -/- or +/+ for washing cells? Thank you

Why was the culture centrifuged after trypsinization and the supernatant discarded?

Thank you for this video series. It helps so much :)

👌

Gold!